Protein A/G

Product               Protein L

Description         

Recombinant Protein A/G contains five Ig-binding regions of protein A and three Ig-binding regions of protein G. Protein A/G binds to all human IgG subclasses and also binds to IgA, IgE, IgM and to a lesser extent IgD; therefore, it has a broader species binding range than either Protein A or Protein G. Unlike non-recombinant Protein G, Protein A/G does not bind serum albumin because the gene sequence coding for the albumin binding site has been removed. Protein A/G is an excellent tool for purification and detection of mouse monoclonal antibodies from IgG subclasses because Protein A/G binds all mouse IgG subclasses but does not bind IgA, IgM or murine serum albumin.

Recombinant Protein A/G from E. coli is produced in validated fermentation and purification processes. In addition, no mammalian components are involved in either the fermentation or downstream processes. The purification process contains several chromatographic steps (but no affinity chromatographic step using human IgG).                           

Main areas of application  

Recombinant Protein A/G interacts with mammalian IgGs mainly through Fc regions.

Working Principle

Protein L has the unique ability to bind kappa light chain interactions without interfering with an antibody's antigen-binding site. This gives ability to bind a wider range of Ig classes and subclasses than other antibody-binding proteins. Protein L will bind to all classes of Ig (IgG, IgM, IgA, IgE and IgD).  Also bind single chain variable fragments (scFv) and Fab fragments. Protein L binds to kappa I, III, and IV in human and kappa I on mouse.

Specifications

Purity                                      ≥ 95%

Production host                     Escherichia coli

Formulation                            20mM NaCl, pH 7.0

Endotoxin                               < 5 EU/mg

Physical state                         Solution

Storage Temperature            ≤ -­20°C

Complies with ISO9001:2015 conditions