Immobilized enzyme development and mass production technology
In order to use expensive enzymes and proteins industrially, these materials should be reused as much as possible in the production process to reduce cost and further increase competitiveness compared to chemical processes.
In order to reuse water-soluble enzymes and proteins, they must be immobilized on an insoluble carrier so that they can be separated from the reaction product.
When the enzymes are immobilized, they can be easily separated from the reaction product and reused continuously. In particular, the stability against external conditions such as temperature, pH, and pressure during the process is significantly increased, and the use cycle can be extended.
However, there are also disadvantages of inactivation due to the modification of the active site of the enzyme, or reduction of the reaction efficiency due to the decrease in material diffusion.
Depending on the type of enzyme and protein used and the reaction system, the type of carrier used for immobilization is different, and the method of immobilization also can be different.
In addition, the immobilized enzyme can be used efficiently when the characteristics including size, pore size, affinity, and strength of the carrier used are optimized according to the reaction system of the enzyme.