Protein G

Product               Protein G

Description         

Protein G is a bacterial cell wall protein originating from group G Streptococci.

Native Protein G has three IgG binding domains and also sites for albumin and cell surface binding. Albumin and cell surface binding domains have been removed from recombinant Protein G to reduce non-specific binding.

Recombinant Protein G from E. coli is produced in validated fermentation and purification processes. In addition, no mammalian components are involved in either the fermentation or downstream processes. 

The purification process contains several chromatographic steps (but no affinity chromatographic step using human IgG).

Main areas of application  

Recombinant Protein G can be used to separate IgG from crude human or mouse IgG serum samples.

Working Principle

Protein G interacts with mammalian IgGs mainly through Fc regions.

Specifications

Purity                                      ≥ 95%

Production host                     Escherichia coli

Formulation                            20mM NaCl, pH 7.0

Endotoxin                               < 5 EU/mg

Physical state                         Solution

Storage Temperature            ≤ -­20°C

Complies with ISO9001:2015 conditions