Protein L

Product               Protein L

Description         

Recombinant Protein L is an immunoglobulin-binding protein that originates from the bacteria Peptostreptococcus magnus. The albumin-binding domain, cell wall and cell membrane binding domains have been eliminated to ensure the maximum specific IgG binding capacity.

Recombinant Protein L from E. coli is produced in validated fermentation and purification processes. In addition, no mammalian components are involved in either the fermentation or downstream processes. The purification process contains several chromatographic steps (but no affinity chromatographic step using human IgG).

Main areas of application: Can be used for purification of free light chains (human kappa I, III, IV and mouse kappa I).

Main areas of application  

Recombinant Protein G can be used to separate IgG from crude human or mouse IgG serum samples.

Working Principle

Protein L has the unique ability to bind kappa light chain interactions without interfering with an antibody's antigen-binding site. This gives ability to bind a wider range of Ig classes and subclasses than other antibody-binding proteins. Protein L will bind to all classes of Ig (IgG, IgM, IgA, IgE and IgD).  Also bind single chain variable fragments (scFv) and Fab fragments. Protein L binds to kappa I, III, and IV in human and kappa I on mouse.

Specifications

Purity                                      ≥ 95%

Production host                     Escherichia coli

Formulation                            20mM NaCl, pH 7.0

Endotoxin                               < 5 EU/mg

Physical state                         Solution

Storage Temperature            ≤ -­20°C

Complies with ISO9001:2015 conditions